EvaluationofAntimicrobialPotentialofSilverNanoparticlesUsingO smiumSanctumFicusBengalensisagainstTwoPathogenicBacteria

Abstract

AgNP has been synthesized from Osmium sanctum and Ficusbenghalenis plants in this
study and tested for antibacterial efficacy against a wide range of microorganisms. The Herbal
approach was used to manufacture AgNP. XRD and FTIR were used to characterize nanoparticles.
Diffusion discs and diffusion wells were used to measure antibacterial activity. Antibacterial activity
in the form of zone of inhibition was discovered in AgNPs. Water and 70% ethanol were used to
dissolve silver nanoparticles (AgNPs). E. coli and Staphylococcus aureus were inhibited by both
solvents containing silver nanoparticles. At 0.4 gm of Osmium sanctum AgNPs + 70% Ethanol, a
maximum inhibition zone of 1.9 cm was obtained against S. aureus using the well diffusion method.
Disk diffusion was used to detect the lowest concentration of FicusbenghalenisAgNPs + water
required to prevent E. coli growth. In the development of antibiotic treatments for various bacterial
illnesses, Osmium sanctum AgNPs have emerged as a key strategy in nanobiotechnology
applications.